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paraformaldehyde  (Thermo Fisher)


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  • 99

    Structured Review

    Thermo Fisher paraformaldehyde
    Paraformaldehyde, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/paraformaldehyde/Paraformaldehyde+Solution%2C+4%25+in+PBS/pmc13284439-129-8-33
    Average 99 stars, based on 1 article reviews
    paraformaldehyde - by Bioz Stars, 2026-09
    99/100 stars

    Images

    Related Articles

    Staining:

    Article Title: Revolutionizing Mg-based guided bone regeneration mesh derived from endogenous dentoalveolar bone augmentation
    Article Snippet: .. The cells were gently rinsed twice with PBS, fixed with 4% paraformaldehyde for 30 min, and then sequentially stained in the dark with Alexa Fluor 488 phalloidin (Invitrogen, USA) for 30 min and DAPI (Invitrogen, USA) for 10 min. Cytoskeletal morphology was imaged using fluorescence microscopy (IX71, Olympus, Japan). ..

    Article Title: Dual-engineering metalloimmunotherapy mediates Staphylococcus aureus virulence silencing and biofilm immune microenvironment reprogramming against implant-associated infections
    Article Snippet: .. Additionally, the dLNs were fixed in paraformaldehyde, embedded in paraffin, and sectioned for immunofluorescence staining of CD80 (Thermo Fisher, USA), CD86, CD4, and CD8 (abcam, USA). ..

    Article Title: Cilostazol inhibits TGF-α-induced migration of hepatocellular carcinoma cells by activating PPARγ.
    Article Snippet: Hepatocellular carcinoma (HCC) is one of the most common cancers worldwide and remains a public health problem with high mortality.. We have previously reported that activation of cyclic adenosine monophosphate (cAMP)-dependent protein kinase (PKA) suppresses transforming growth factor (TGF)-α-induced migration of HCC cells.. In the present study, we investigated whether cilostazol, a phosphodiesterase (PDE) inhibitor, affects the TGF-α-induced migration of HCC cells.

    Article Title: Preclinical Drug-Response Profiling Identifies BMI1 Inhibition as a Therapeutic Option for Hepatoblastoma.
    Article Snippet: Cell proliferation was assessed using the Click-iT EdU Imaging Kit (Thermo Fisher, Waltham, MA, USA) following the manufacturer’s protocol. .. Briefly, 2 × 105 cells were seeded into 24-well plates and incubated in 100 μM ethynyl-deoxyuridine (EdU) for 24 h. Then, cells were treated with 250 nM PTC209, 250 nM PTC596, or DMSO for an additional 24 h. Subsequently, cells were fixed with 3.7% paraformaldehyde, permeabilized with 0.5% Triton X-100 in PBS, and stained with Hoechst 33342 (Thermo Fisher) along with the Click-iT staining cocktail containing reaction buffer, CuSO4, and Alexa Fluor azide (Thermo Fisher). .. Images were acquired using the EVOS M7000 imaging system (Thermo Fisher).

    Fluorescence:

    Article Title: Revolutionizing Mg-based guided bone regeneration mesh derived from endogenous dentoalveolar bone augmentation
    Article Snippet: .. The cells were gently rinsed twice with PBS, fixed with 4% paraformaldehyde for 30 min, and then sequentially stained in the dark with Alexa Fluor 488 phalloidin (Invitrogen, USA) for 30 min and DAPI (Invitrogen, USA) for 10 min. Cytoskeletal morphology was imaged using fluorescence microscopy (IX71, Olympus, Japan). ..

    Microscopy:

    Article Title: Revolutionizing Mg-based guided bone regeneration mesh derived from endogenous dentoalveolar bone augmentation
    Article Snippet: .. The cells were gently rinsed twice with PBS, fixed with 4% paraformaldehyde for 30 min, and then sequentially stained in the dark with Alexa Fluor 488 phalloidin (Invitrogen, USA) for 30 min and DAPI (Invitrogen, USA) for 10 min. Cytoskeletal morphology was imaged using fluorescence microscopy (IX71, Olympus, Japan). ..

    Incubation:

    Article Title: MCL inhibits macrophage ferroptosis through the NRF2/HO-1/GPX4 axis to attenuate carotid atherosclerotic plaque formation.
    Article Snippet: Background: Micheliolide (MCL) exhibits strong antioxidant and anti-inflammatory properties.. Carotid atherosclerotic plaques are a key risk factor for ischemic stroke.. This study investigates MCL's protective effects on carotid atherosclerosis and its mechanism.

    Article Title: Combined spermidine and umbilical cord mesenchymal stem cell therapy repairs endometrial injury in mice via IL-10/Treg-Dependent immune microenvironment modulation.
    Article Snippet: The aim of the present study was to explore the reparative effects of spermidine combined with umbilical cord mesenchymal stem cells (UC-MSCs) on endometrial injury in mice.. First, bioinformatic results suggested that endometrial damage is closely linked to immune mechanisms, a finding that was corroborated by quantitative polymerase chain reaction results.. Next, an endometrial injury model was established by injecting 95% ethanol into the uterus, and spermidine combined with UC-MSCs was used for treatment.

    Article Title: Preclinical Drug-Response Profiling Identifies BMI1 Inhibition as a Therapeutic Option for Hepatoblastoma.
    Article Snippet: Cell proliferation was assessed using the Click-iT EdU Imaging Kit (Thermo Fisher, Waltham, MA, USA) following the manufacturer’s protocol. .. Briefly, 2 × 105 cells were seeded into 24-well plates and incubated in 100 μM ethynyl-deoxyuridine (EdU) for 24 h. Then, cells were treated with 250 nM PTC209, 250 nM PTC596, or DMSO for an additional 24 h. Subsequently, cells were fixed with 3.7% paraformaldehyde, permeabilized with 0.5% Triton X-100 in PBS, and stained with Hoechst 33342 (Thermo Fisher) along with the Click-iT staining cocktail containing reaction buffer, CuSO4, and Alexa Fluor azide (Thermo Fisher). .. Images were acquired using the EVOS M7000 imaging system (Thermo Fisher).

    Article Title: Commensal microbiota–coated biohybrid implants induce antibiofilm, osteogenic, and immunomodulatory responses in a human 3D immunocompetent model
    Article Snippet: .. After 24 h, cells were fixed with 4% paraformaldehyde, washed with Tris-buffered saline (TBS), and blocked with 3% goat serum containing 0.1% Tween 20 for 30 min. Primary antibodies - (rabbit polyclonal anti-CD80, Invitrogen) for M1 and (mouse monoclonal anti- CD206, clone 2A6A10, Proteintech) for M2-were then applied at the appropriate dilution and incubated overnight at 4°C with gentle shaking. .. The following day, the antibody solution was removed, and the samples were washed prior to incubation with secondary antibodies - Alexa FluorTM 488-conjugated goat anti-rabbit IgG (H + L) (Invitrogen) and CyTM5-conjugated AffiniPureTM donkey anti-mouse IgG (H + L) (Jackson ImmunoResearch Laboratories Inc., USA) for 2.5 h. Samples were subsequently washed, counterstained with DAPI, and imaged by CLSM.

    Article Title: Capsaicin as a Phytochemical Adjuvant Enhancing Cisplatin Efficacy in Nsclc: In Vitro and In Vivo Evidence.
    Article Snippet: .. After 48 h of incubation, the cells were washed three times with phosphate-buffered saline (PBS; Thermo Fisher Scientific, 10010023, Waltham, MA, USA) and fixed with 4% paraformaldehyde (Thermo Fisher Scientific, J62478.AK, Waltham, MA, USA) for 15 min at room temperature. .. Permeabilization was performed using 0.1% Triton X-100 (Thermo Fisher Scientific, HFH10, Waltham, MA, USA) for 10 min, followed by blocking with a commercial blocking reagent (Vector Laboratories, MP7401, Newark, CA, USA) for 20 min to minimize nonspecific antibody binding.

    Immunofluorescence:

    Article Title: Dual-engineering metalloimmunotherapy mediates Staphylococcus aureus virulence silencing and biofilm immune microenvironment reprogramming against implant-associated infections
    Article Snippet: .. Additionally, the dLNs were fixed in paraformaldehyde, embedded in paraffin, and sectioned for immunofluorescence staining of CD80 (Thermo Fisher, USA), CD86, CD4, and CD8 (abcam, USA). ..

    Membrane:

    Article Title: Cilostazol inhibits TGF-α-induced migration of hepatocellular carcinoma cells by activating PPARγ.
    Article Snippet: Hepatocellular carcinoma (HCC) is one of the most common cancers worldwide and remains a public health problem with high mortality.. We have previously reported that activation of cyclic adenosine monophosphate (cAMP)-dependent protein kinase (PKA) suppresses transforming growth factor (TGF)-α-induced migration of HCC cells.. In the present study, we investigated whether cilostazol, a phosphodiesterase (PDE) inhibitor, affects the TGF-α-induced migration of HCC cells.

    Saline:

    Article Title: Commensal microbiota–coated biohybrid implants induce antibiofilm, osteogenic, and immunomodulatory responses in a human 3D immunocompetent model
    Article Snippet: .. After 24 h, cells were fixed with 4% paraformaldehyde, washed with Tris-buffered saline (TBS), and blocked with 3% goat serum containing 0.1% Tween 20 for 30 min. Primary antibodies - (rabbit polyclonal anti-CD80, Invitrogen) for M1 and (mouse monoclonal anti- CD206, clone 2A6A10, Proteintech) for M2-were then applied at the appropriate dilution and incubated overnight at 4°C with gentle shaking. .. The following day, the antibody solution was removed, and the samples were washed prior to incubation with secondary antibodies - Alexa FluorTM 488-conjugated goat anti-rabbit IgG (H + L) (Invitrogen) and CyTM5-conjugated AffiniPureTM donkey anti-mouse IgG (H + L) (Jackson ImmunoResearch Laboratories Inc., USA) for 2.5 h. Samples were subsequently washed, counterstained with DAPI, and imaged by CLSM.

    Article Title: Capsaicin as a Phytochemical Adjuvant Enhancing Cisplatin Efficacy in Nsclc: In Vitro and In Vivo Evidence.
    Article Snippet: .. After 48 h of incubation, the cells were washed three times with phosphate-buffered saline (PBS; Thermo Fisher Scientific, 10010023, Waltham, MA, USA) and fixed with 4% paraformaldehyde (Thermo Fisher Scientific, J62478.AK, Waltham, MA, USA) for 15 min at room temperature. .. Permeabilization was performed using 0.1% Triton X-100 (Thermo Fisher Scientific, HFH10, Waltham, MA, USA) for 10 min, followed by blocking with a commercial blocking reagent (Vector Laboratories, MP7401, Newark, CA, USA) for 20 min to minimize nonspecific antibody binding.

    Gentle:

    Article Title: Commensal microbiota–coated biohybrid implants induce antibiofilm, osteogenic, and immunomodulatory responses in a human 3D immunocompetent model
    Article Snippet: .. After 24 h, cells were fixed with 4% paraformaldehyde, washed with Tris-buffered saline (TBS), and blocked with 3% goat serum containing 0.1% Tween 20 for 30 min. Primary antibodies - (rabbit polyclonal anti-CD80, Invitrogen) for M1 and (mouse monoclonal anti- CD206, clone 2A6A10, Proteintech) for M2-were then applied at the appropriate dilution and incubated overnight at 4°C with gentle shaking. .. The following day, the antibody solution was removed, and the samples were washed prior to incubation with secondary antibodies - Alexa FluorTM 488-conjugated goat anti-rabbit IgG (H + L) (Invitrogen) and CyTM5-conjugated AffiniPureTM donkey anti-mouse IgG (H + L) (Jackson ImmunoResearch Laboratories Inc., USA) for 2.5 h. Samples were subsequently washed, counterstained with DAPI, and imaged by CLSM.



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